Wet Lab

• Bacterial Culture - Measuring Optical Density: How to measure bacterial culture concentration (optical density)

• Bacterial Culture - Serial Dilution: How to thaw CaCo2 cells from frozen and start a culture in a flask.

• Caco-2 Cell Counting: How to know when to count cells and how to count them.

• Caco-2 Changing Cell Culture Media from a Flask: How to know when to change cell culture media from a flask and how to change

• Caco-2 Changing Cell Culture Media of a Transwell Monolayer: How to know when to change cell culture media in a Transwell monolayer and how to change

• Caco-2 Media Recipe: The recipe for the DMEM CaCo2 cell culture growth medium.

• Caco-2 Splitting Cells: How to split CaCo2 cells once they are confluent.

• Caco-2 Starting a Frozen Stock: How to start a frozen stock when passaging cells

• Caco-2 Thawing Cells From Frozen: How to thaw CaCo2 cells from frozen and start a culture in a flask.