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Serial Dilution

Use this page when completing a Serial Dilution of a bacterial culture, trying to reach a certain concentration of bacteria for experiments.ss

Purpose

Serial Dilution is done when you want to confirm accurately the number of bacteria in a certain bacterial culture. It is essential to ensure that all results from experiments using a bacterial culture are accurate and informed.

Safety

• Spray down all working materials with 70% Ethanol.
• Try not to touch the sides of tubes with pipette tips.
• Do not touch the rims of the flasks or tubes to work with.

Materials

Procedure

(Caco-2 media recipe)

1. Prepare bacterial culture link
2. After washing the bacteria with PBS and a suspended bacterial-PBS solution was made,

Quality Control

• Check the clarity of the supernatant and the visibility of the pellet.
• Make sure to complete the methods quickly and accurately.

Revision History